Chicken Serotonin/5 Hydroxytryptamine ELISA Kit | 5-HT elisa kit

Chicken Serotonin/5 Hydroxytryptamine ELISA Kit

Cat. Num. AAA17020

• Reactivity: Chicken
• Synonyms: Serotonin/5 Hydroxytryptamine; N/A; Chicken Serotonin/5 Hydroxytryptamine ELISA Kit; 5-HT elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of 5HT. No significant cross-reactivity or interference between 5HT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between 5HT and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for 5-HT elisa kit

Principle of the Assay: 5HT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-5HT antibody and an 5HT-HRP conjugate. The assay sample and buffer are incubated together with 5HT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 5HT concentration since 5HT from samples and 5HT-HRP conjugate compete for the anti-5HT antibody binding site. Since the number of sites is limited, as more sites are occupied by 5HT from the sample, fewer sites are left to bind 5HT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 5HT concentration in each sample is interpolated from this standard curve.

Intended Uses: This 5HT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken 5HT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for 5-HT elisa kit

Neurobiology