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Mouse anti-Human SMAD1 Monoclonal Antibody | anti-SMAD1 antibody
Mouse monoclonal antibody Anti-Human SMAD1
Gene Names
SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
Reactivity
Human
Applications
Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry, Functional Assay
Synonyms
SMAD1, Antibody; Mouse monoclonal antibody Anti-Human SMAD1; Homo sapiens SMAD family member 1 (SMAD1), transcript variant 1; BSP1; JV41; JV4-1;MADH1; MADR1; anti-SMAD1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
913C1b
Concentration
100 ug/ml (1.0 ml) (varies by lot)
Sequence Length
3056
Applicable Applications for anti-SMAD1 antibody
Dot Blot (DB), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)
Preparation
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
Sterility
Filtered through a 0.22 um membrane.
ICC (Immunocytochemistry)
(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-SMAD1 mAb. [Lot No. 913C1b-1])
FCM (Flow Cytometry)
(HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-SMAD1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 913C1b-1])
IP (Immunoprecipitation)
(Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-SMAD1 mAb. [Lot No. 913C1b-1]Predicted molecular weight: 52 kDa)
WB (Western Blot)
(Detection of SMAD1 by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 913C1b-1]Predicted molecular weight: 52 kDa)
Quality Control
(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-SMAD1 monoclonal antibody.)
Related Product Information for anti-SMAD1 antibody
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [NCBI Entrez Gene Summary]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,406 Da
NCBI Official Full Name
Homo sapiens SMAD family member 1 (SMAD1), transcript variant 1, mRNA
NCBI Official Synonym Full Names
SMAD family member 1
NCBI Official Symbol
SMAD1
NCBI Official Synonym Symbols
BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
NCBI Protein Information
mothers against decapentaplegic homolog 1; MAD homolog 1; MAD, mothers against decapentaplegic homolog 1; Mad-related protein 1; SMAD, mothers against DPP homolog 1; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; transforming growth factor-beta-signaling protein 1
UniProt Protein Name
Mothers against decapentaplegic homolog 1
UniProt Gene Name
SMAD1
UniProt Entry Name
SMAD1_HUMAN
