Rabbit TFF3 Polyclonal Antibody | anti-TFF3 antibody

TFF3 Antibody

Cat. Num. AAA10911

• Gene Names: TFF3; ITF; P1B; TFI
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: TFF3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TFF3; Polyclonal Antibody; TFF3 Antibody; ITF; P1B; TFI; Trefoil factor 3; Intestinal trefoil factor; hITF; trefoil factor 3 (intestinal); anti-TFF3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TFF3 antibody is predicted to not cross-react with other TFF family members.
• Purity/Purification: TFF3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 137

• Applicable Applications for anti-TFF3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: TFF3 antibody can be used for detection of TFF3 by Western blot at 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TFF3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TFF3.
• Buffer: TFF3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TFF3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IHC (Immunohistchemistry)

(Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of TFF3 in human small intestine tissue with TFF3 antibody at 20 μg/ml.Red: TFF3 Antibody (6113)Blue: DAPI staining)

ICC (Immunocytochemistry)

(Immunocytochemistry of TFF3 in HeLa cells with TFF3 antibody at 2 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of TFF3 in Human Small Intestine tissue with TFF3 antibody at 20 μg/mL.)

IHC (Immunohistochemistry)

(Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2.5 μg/mL.)

WB (Western Blot)

(Western blot analysis of TFF3 in human colon tissue lysate with TFF3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Related Product Information for anti-TFF3 antibody

TFF3 Antibody: Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.

NCBI and Uniprot Product Information

• NCBI GI #: 119629977
• NCBI GeneID: 7033
• UniProt Accession #: Q07654; Q96NX0; Q9UDA5; E9PBB5
• Molecular Weight: Predicted: 10 kDa; ; Observed: 11 kDa
• NCBI Official Full Name: trefoil factor 3 (intestinal), isoform CRA_b
• NCBI Official Synonym Full Names: trefoil factor 3 (intestinal)
• NCBI Official Symbol: TFF3
• NCBI Official Synonym Symbols: ITF; P1B; TFI
• NCBI Protein Information: trefoil factor 3; polypeptide P1.B
• UniProt Protein Name: Trefoil factor 3
• UniProt Gene Name: TFF3
• UniProt Synonym Gene Names: ITF; TFI; hITF; hP1.B
• UniProt Entry Name: TFF3_HUMAN