Rabbit Caspase-12 Polyclonal Antibody | anti-Casp12 antibody

Caspase-12 Antibody (Large)

Cat. Num. AAA10958

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Caspase-12 (Large) Antibody is affinity chromatography purified via peptide column.
• Synonyms: Caspase-12, Antibody; Caspase-12 Antibody (Large); Caspase-12 (Large); Caspase-12; CASP-12; caspase 12; anti-Casp12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Caspase-12 (large) antibody is predicted to have no cross reactivity to other members in the caspase family.
• Purity/Purification: Caspase-12 (Large) Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 419

• Applicable Applications for anti-Casp12 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Caspase-12 (large) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 0.5 to 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Caspase-12 antibody was raised against a synthetic peptide corresponding to 16 amino acids within the large cleavage product of murine caspase-12.
• Buffer: Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Large)
• Preparation and Storage: Caspase-12 (Large) antibody can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of Caspase-12 in mouse heart tissue with Caspase-12 antibody at 20 μg/ml.Green: Caspase-12 Antibody (Large) (3195)Blue: DAPI staining)

IHC (Immunohistchemistry)

(Immunohistochemistry of Caspase-12 in mouse heart tissue with Caspase-12 antibody at 2 μg/ml.)

WB (Western Blot)

(Western blot analysis of Caspase-12 expression in rat heart tissue lysate with Caspase-12 antibody at (A) 0.25 and (B) 0.5 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of Caspase-12 in rat heart tissue with Caspase-12 antibody at 20 μg/ml.)

IHC (Immunohistochemistry)

(Immunohistochemistry of Caspase-12 in rat heart tissue with Caspase-12 antibody at 2.5 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of Caspase-12 in Human Heart tissue with Caspase-12 antibody at 10 μg/mL.)

IHC (Immunohistochemistry)

(Immunohistochemical staining of human heart tissue using caspase-12 antibody at 2 μg/mL.)

Related Product Information for anti-Casp12 antibody

Caspase-12 Antibody: (Large)Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 2094806
• NCBI GeneID: 12364
• UniProt Accession #: O08736; Q3TT82
• Molecular Weight: Predicted: 46 kDa; ; Observed: 46 kDa
• NCBI Official Full Name: caspase-12
• NCBI Official Synonym Full Names: caspase 12
• NCBI Official Symbol: Casp12
• NCBI Protein Information: caspase-12; CASP-12
• UniProt Protein Name: Caspase-12
• UniProt Gene Name: Casp12
• UniProt Synonym Gene Names: CASP-12
• UniProt Entry Name: CASPC_MOUSE