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WB (Western Blot)
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Rabbit SQSTM1 Polyclonal Antibody | anti-SQSTM1 antibody

SQSTM1 Antibody

Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
SQSTM1 Antibody is affinity chromatography purified via peptide column.
Synonyms
SQSTM1, Antibody; SQSTM1 Antibody; SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B; ORCA; Sequestosome-1; EBI3-associated protein of 60 kDa; EBIAP; sequestosome 1; anti-SQSTM1 antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
SQSTM1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
440
Applicable Applications for anti-SQSTM1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
SQSTM1 antibody can be used for detection of SQSTM1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
SQSTM1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human SQSTM1.
Buffer
SQSTM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.

WB (Western Blot)

(Western blot analysis of SQSTM1 expression in K562 cell lysate with AP3B2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

IF (Immunofluorescence)

(Immunofluorescence of SQSTM1 in mouse spleen tissue with SQSTM1 antibody at 20 μg/ml.Green: SQSTM1 Antibody (5449)Blue: DAPI staining)

IF (Immunofluorescence)

(Immunofluorescence of SQSTM1 in A431 cells with SQSTM1 antibody at 20 μg/ml.Green: SQSTM1 Antibody (5449)Blue: DAPI staining)

IHC (Immunohistochemistry)

(Immunohistochemistry of SQSTM1 in mouse spleen tissue with SQSTM1 antibody at 2 μg/ml.)

IHC (Immunohistochemistry)

(Immunohistochemistry of SQSTM1 in human spleen tissue with SQSTM1 antibody at 5 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of SQSTM1 in Rat Spleen cells with SQSTM1 antibody at 20 μg/mL.)

IHC (Immunohistochemistry)

(Immunohistochemistry of SQSTM1 in rat spleen tissue with SQSTM1 antibody at 5 μg/mL.)

WB (Western Blot)

(Western blot analysis of SQSTM1 in Human spleen tissue lysate with SQSTM1 antibody at (A) 1 and (B) 2 μg/mL.)

Related Product Information for anti-SQSTM1 antibody
SQSTM1 Antibody: SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-κB by TNF-α, nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1's UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget's disease of bone since the UBA is necessary for aggregate sequestration and cell survival.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
38,629 Da
NCBI Official Full Name
Sequestosome-1
NCBI Official Synonym Full Names
sequestosome 1
NCBI Official Symbol
SQSTM1
NCBI Official Synonym Symbols
p60; p62; A170; OSIL; PDB3; ZIP3; p62B
NCBI Protein Information
sequestosome-1; EBIAP; EBI3-associated protein p60; oxidative stress induced like; ubiquitin-binding protein p62; EBI3-associated protein of 60 kDa; phosphotyrosine independent ligand for the Lck SH2 domain p62; phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
UniProt Protein Name
Sequestosome-1
UniProt Gene Name
SQSTM1
UniProt Synonym Gene Names
ORCA; OSIL; EBIAP; p60
UniProt Entry Name
SQSTM_HUMAN

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Product Notes

The SQSTM1 sqstm1 (Catalog #AAA10960) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SQSTM1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SQSTM1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). SQSTM1 antibody can be used for detection of SQSTM1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the SQSTM1 sqstm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SQSTM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.