Rabbit Vascular Endothelial Cell Growth Factor ELISA Kit | VEGF elisa kit
Rabbit Vascular Endothelial Cell Growth Factor ELISA Kit
Reactivity
Rabbit
Synonyms
Vascular Endothelial Cell Growth Factor; N/A; Rabbit Vascular Endothelial Cell Growth Factor ELISA Kit; VEGF elisa kit
Reactivity
Rabbit
Specificity
Specifically recognize VEGF, no obvious cross reaction with other analogues
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
1.563-100pg/ml
Sensitivity
0.938pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for VEGF elisa kit
Background: Vascular Endothelial Growth Factor (VEGF) is a growth factor that plays an important role in angiogenesis and regulation of vascular permeability. It promotes the proliferation and migration of endothelial cells and also stimulates angiogenesis and maintains mature vascular networks. The function of VEGF is involved in many physiological and pathological processes, including embryonic development, tumor growth and metastasis, inflammation, heart disease, and neurological diseases. VEGF has several different subtypes, including VEGF-A, VEGF-B, VEGF-C, VEGF-D, and VEGF-E. Among them, VEGF-A is the most extensively studied subtype and has various spliced variants with different biological functions. VEGF-A acts by binding to VEGF receptors, such as VEGFR1 and VEGFR2.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti VEGF antibody was precoated onto the 96-well plate. The biotin conjugated anti VEGF antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with VEGF conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of VEGF in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti VEGF antibody was precoated onto the 96-well plate. The biotin conjugated anti VEGF antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with VEGF conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of VEGF in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
