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Mouse anti-Human CTLA-4 Monoclonal Antibody | anti-CTLA4 antibody

CTLA-4 Antibody [1E6]

Gene Names
CTLA4; CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A purified IgG1.
Synonyms
CTLA-4, Antibody; CTLA-4 Antibody [1E6]; CTLA-4 Antibody; CTL4; cytotoxic T-lymphocyte associated protein 4; CD; GSE; GRD4; ALPS5; CD152; IDDM12; CELIAC3; anti-CTLA4 antibody
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For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Purity/Purification
Protein A purified IgG1.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
223
Applicable Applications for anti-CTLA4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Western blot at 0.125 - 0.25 mug/mL.
Immunohistochemistry starting at 5 mug/mL.
Immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.
Buffer
CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide.
Species
Human
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.

ELISA

(Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with antibodies at decreasing concentrations.)

IHC (Immunohistochemistry)

(Immunohistochemistry of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 5 μg/mL.)

IHC (Immunohistchemistry)

(Immunohistochemistry of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 5 μg/mL.)

IF (Immunofluorescence)

(Immunofluorescence of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 20 μg/mL.Red: CTLA4 Antibody [1E6]Blue: DAPI staining)

IF (Immunofluorescence)

(Immunofluorescence of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 20 μg/mL.Red: CTLA4 Antibody [1E6]Blue: DAPI staining)

IF (Immunofluorescence)

(Immunofluorescence of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 20 μg/mL.Red: CTLA4 Antibody [1E6]Blue: DAPI staining)

ICC (Immunocytochemistry)

(Immunocytochemistry of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.)

WB (Western Blot)

(Western blot analysis of CTLA-4 in overexpressing HEK293 cells CTLA-4 antibody at 0.125 and 0.25 μg/mL.)

Related Product Information for anti-CTLA4 antibody
The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2). Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3). Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4). CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5).
Product Categories/Family for anti-CTLA4 antibody
References
1. Brunet JF, Denizot F, Luciani MF, et al. A new member of the immunoglobulin superfamily--CTLA-4. Nature 1987; 328:267-70. 2. Walunas TL, Lenschow DJ, Bakker CY, et al. CTLA-4 can function as a negative regulator of T cell activation. Immunity 1994; 1:405-13. 3. Harding FA, McArthur JG, Gross JA, et al. CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature 1992; 356:607-9. 4. Romo-Tena J, Gomez-Martin D, and Alcocer-Verela J. CTLA-4 and autoimmunity: new insights into the dual regulator of tolerance. Autoimmun. Rev. 2013; 12:117.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted: 25 kDa
Observed: 32 kDa
NCBI Official Full Name
cytotoxic T-lymphocyte protein 4 isoform CTLA4-TM
NCBI Official Synonym Full Names
cytotoxic T-lymphocyte associated protein 4
NCBI Official Symbol
CTLA4
NCBI Official Synonym Symbols
CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
NCBI Protein Information
cytotoxic T-lymphocyte protein 4
UniProt Protein Name
Cytotoxic T-lymphocyte protein 4
UniProt Gene Name
CTLA4
UniProt Synonym Gene Names
CD152; CTLA-4

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Product Notes

The CTLA4 ctla4 (Catalog #AAA10982) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CTLA-4 Antibody [1E6] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CTLA-4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF). Western blot at 0.125 - 0.25 mug/mL. Immunohistochemistry starting at 5 mug/mL. Immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the CTLA4 ctla4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTLA-4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.