IHC (Immunohistochemistry)
(Figure 7. Immunohistochemistry Validation of ZC3H12A in Rat BrainImmunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody (AAA11042) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
Rabbit ZC3H12A Polyclonal Antibody | anti-ZC3H12A antibody
ZC3H12A (CT) Antibody
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples; Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
The immunogen is located within the last 50 amino acids of ZC3H12A.
IHC (Immunohistochemistry)
(Figure 7. Immunohistochemistry Validation of ZC3H12A in Rat BrainImmunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody (AAA11042) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistochemistry)
(Figure 7. Immunohistochemistry Validation of ZC3H12A in Rat BrainImmunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ZC3H12A antibody (AAA11042) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistchemistry)
(Figure 6. Immunohistochemistry Validation of ZC3H12A in Mouse LiverImmunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ZC3H12A antibody (AAA11042) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin)
IHC (Immunohistchemistry)
(Figure 6. Immunohistochemistry Validation of ZC3H12A in Mouse LiverImmunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ZC3H12A antibody (AAA11042) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin)
IHC (Immunohistochemistry)
(Figure 5. Immunohistochemistry Validation of ZC3H12A in Human TestisImmunohistochemical analysis of paraffin-embedded human testis tissue using anti-ZC3H12A antibody (AAA11042) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistochemistry)
(Figure 5. Immunohistochemistry Validation of ZC3H12A in Human TestisImmunohistochemical analysis of paraffin-embedded human testis tissue using anti-ZC3H12A antibody (AAA11042) at 1ug/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IF (Immunofluorescence)
(Figure 4. Immunofluorescence Validation of ZC3H12A in Human THP-1 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed THP-1 cells labeling ZC3H12A with AAA11042 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 4. Immunofluorescence Validation of ZC3H12A in Human THP-1 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed THP-1 cells labeling ZC3H12A with AAA11042 at 10ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
WB (Western Blot)
(Figure 3. Western Blot Validation in Rat Tissues Loading: 15ug of lysates per lane. Antibodies: ZC3H12A AAA11042, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 3. Western Blot Validation in Rat Tissues Loading: 15ug of lysates per lane. Antibodies: ZC3H12A AAA11042, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 2 Western Blot Validation in Mouse Tissues Loading: 15ug of lysates per lane. Antibodies: ZC3H12A AAA11042, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 2 Western Blot Validation in Mouse Tissues Loading: 15ug of lysates per lane. Antibodies: ZC3H12A AAA11042, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 1. WB Validation in Human Lung Loading: 15ug of lysate per lane. Antibodies: ZC3H12A AAA11042, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2ug/mL Lane 2: 4ug/mL)
WB (Western Blot)
(Figure 1. WB Validation in Human Lung Loading: 15ug of lysate per lane. Antibodies: ZC3H12A AAA11042, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: 2ug/mL Lane 2: 4ug/mL)
Nowak et al. RNA Biol. 2021; 18(1):144-156
Tyka et al. Biochim Biophys Acta Mol Basis Dis. 2021; 1867(10):166199
Boratyn et al. J Cell Biochem. 2020; 121(5-6);3406-3425
NCBI and Uniprot Product Information
Observed: 66 kDa
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Product Notes
The ZC3H12A zc3h12a (Catalog #AAA11042) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ZC3H12A (CT) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ZC3H12A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC). WB: 2ug mL; IF: 10ug mL; IHC: 1ug mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human samples; Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the ZC3H12A zc3h12a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZC3H12A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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