Rabbit PEX3 Polyclonal Antibody | anti-PEX3 antibody

PEX3 (IN) Antibody

Cat. Num. AAA11048

• Gene Names: PEX3; TRG18; PBD10A
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence
• Purity: PEX3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: PEX3, Antibody; PEX3 (IN) Antibody; PEX3 Antibody: Peroxisomal biogenesis factor 3; Peroxin-3; Peroxisomal assembly protein PEX3; anti-PEX3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: PEX3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; PEX3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-PEX3 antibody: ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 2ug/mL; IF: 20ug/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
• Immunogen: Anti-PEX3 antibody (9667) was raised against a peptide corresponding to 14 amino acids near the center of human PEX3. The immunogen is located within the center of PEX3.
• Homology: Predicted species reactivity based on immunogen sequence: monkey (100%), cow (100%), chicken (100%)
• Research Area: Signal Transduction
• NCBI Organism: Homo sapiens
• Isoforms: Human PEX3 has 1 isoform, (373aa, 42kD). Mouse PEX3 has 1 isoform (372aa, 42kD). Rat PEX3 has one isoform (372aa, 42kD). can detect PEX3 in human, mouse and rat.
• Preparation and Storage: PEX3 antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Figure 7 Immunofluorescence Validation of PEX3 in Rat testisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX3 with AAA11048 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence)

(Figure 6 Immunofluorescence Validation of PEX3 in Mouse ThymusImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX3 with AAA11048 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence)

(Figure 5 Immunofluorescence Validation of PEX3 in Human SpleenImmunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX3 with AAA11048 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence)

(Figure 4 Immunofluorescence Validation of PEX3 in Human HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PEX3 with AAA11048 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

WB (Western Blot)

(Figure 3 Western Blot Validation in Rat TissuesLoading: 15ug of lysates per lane.Antibodies: PEX3 AAA11048, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

WB (Western Blot)

(Figure 2 Western Blot Validation in Mouse Tissues Loading: 15ug of lysates per lane.Antibodies: PEX3 AAA11048, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

WB (Western Blot)

(Figure 1 WB Validation in Human Cell Lines Loading: 15ug of lysate Antibodies: PEX3 AAA11048, 2ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.)

Related Product Information for anti-PEX3 antibody

PEX3 Antibody: PEX3 is involved in peroxisome biosynthesis and integrity. It assembles membrane vesicles before the matrix proteins are translocated. As a docking factor for PEX19, PEX3 is necessary for the import of peroxisomal membrane proteins in the peroxisomes. Thus within the cell, PEX3 is stabilized by PEX19 preventing PEX3 aggregation. Mutations in the PEX19-binding domain of PEX3 reduce the affinity for PEX19 and destabilize PEX3.

Product Categories/Family for anti-PEX3 antibody

Primary Antibodies

References

Ghaedi et al. Mol Biol Cell. 2000; 11(6):2085-2102
Fang et al. J Cell Biol. 2004;164(6):863-875
Hattula et al. PloS One. 2014; 9(7):e103101
Schmidt et al. Traffic. 2012; 13(9):1244-1260

NCBI and Uniprot Product Information

• NCBI GI #: 3914303
• NCBI GeneID: 8504
• NCBI Accession #: NP_003621.1
• NCBI GenBank Nucleotide #: NM_003630.2
• UniProt Accession #: P56589; Q6FGP5
• Molecular Weight: 42,140 Da
• NCBI Official Full Name: peroxisomal biogenesis factor 3
• NCBI Official Synonym Full Names: peroxisomal biogenesis factor 3
• NCBI Official Symbol: PEX3
• NCBI Official Synonym Symbols: TRG18; PBD10A
• NCBI Protein Information: peroxisomal biogenesis factor 3; peroxin-3; peroxisomal assembly protein PEX3; transformation-related protein 18
• UniProt Protein Name: Peroxisomal biogenesis factor 3
• UniProt Gene Name: PEX3
• UniProt Entry Name: PEX3_HUMAN