Sheep Coagulation Factor XII ELISA Kit | F12 elisa kit
Sheep Coagulation Factor XII ELISA Kit
Gene Names
F12; FXII
Reactivity
Sheep
Synonyms
Coagulation Factor XII; N/A; Sheep Coagulation Factor XII ELISA Kit; F12 elisa kit
Reactivity
Sheep
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for F12 elisa kit
Intended Uses: This FXa ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FXa. This ELISA kit for research use only!
Principle of the Assay: FXa ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FXa antibody and an FXa-HRP conjugate. The assay sample and buffer are incubated together with FXa-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FXa concentration since FXa from samples and FXa-HRP conjugate compete for the anti-FXa antibody binding site. Since the number of sites is limited, as more sites are occupied by FXa from the sample, fewer sites are left to bind FXa-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FXa concentration in each sample is interpolated from this standard curve.
Principle of the Assay: FXa ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FXa antibody and an FXa-HRP conjugate. The assay sample and buffer are incubated together with FXa-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FXa concentration since FXa from samples and FXa-HRP conjugate compete for the anti-FXa antibody binding site. Since the number of sites is limited, as more sites are occupied by FXa from the sample, fewer sites are left to bind FXa-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FXa concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
68,012 Da
NCBI Official Full Name
FXII
NCBI Official Symbol
F12
NCBI Official Synonym Symbols
FXII
NCBI Protein Information
coagulation factor XII; HAF; hageman factor
UniProt Protein Name
Coagulation factor XII
UniProt Gene Name
F12
UniProt Synonym Gene Names
HAF
UniProt Entry Name
FA12_PIG
