Reactivity
Human
Sequence Length
260
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.5ng/ml - 200ng/ml
Sensitivity
0.24ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for MYOC elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Myocilin (also known as MYOC) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human MYOC antibody. MYOC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human MYOC Antibody is added and binds to MYOC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MYOCantibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human MYOC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human MYOC antibody. MYOC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human MYOC Antibody is added and binds to MYOC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MYOCantibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human MYOC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
29,494 Da
NCBI Official Full Name
myocilin, partial
UniProt Protein Name
Myocilin
UniProt Gene Name
MYOC