Human cluster Of differentiation, CD4 ELISA Kit | CD4 elisa kit

Human cluster Of differentiation, CD4 ELISA Kit

Cat. Num. AAA11292

• Reactivity: Human
• Synonyms: cluster Of differentiation, CD4; N/A; Human cluster Of differentiation, CD4 ELISA Kit; CD4 elisa kit

• Reactivity: Human
• Assay Type: Quantitative Sandwich
• Samples: Serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids
• Detection Range: 0.1-30ng/ml
• Sensitivity: 0.05ng/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Standard Curve (Sample)

Related Product Information for CD4 elisa kit

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CD4 antibody. CD4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD4 Antibody is added and binds to CD4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CD4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human cluster Of differentiation 4 (also known as CD4) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.