Reactivity
Human
Sequence Length
731
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
3ng/ml - 900ng/ml
Sensitivity
1.58ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for GS elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Gelsolin (also known as GS) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human GS antibody. GS present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human GS Antibody is added and binds to GS in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GS antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human GS. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human GS antibody. GS present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human GS Antibody is added and binds to GS in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GS antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human GS. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,827 Da
NCBI Official Full Name
gelsolin
NCBI Official Symbol
GSN
NCBI Official Synonym Symbols
ADF
NCBI Protein Information
gelsolin
UniProt Protein Name
Gelsolin
UniProt Gene Name
GSN
UniProt Synonym Gene Names
ADF