CFI recombinant protein

Human CFI Protein, His Tag

Cat. Num. AAA11715

• Gene Names: CFI; FI; IF; KAF; AHUS3; C3BINA; C3b-INA
• Purity: Purified from cell culture supernatant by affinity chromatography; The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
• Synonyms: CFI; Human CFI Protein; His Tag; FI; IF; KAF; AHUS3; ARMD13; C3BINA; C3b-INA; CFI recombinant protein

• Host: HEK293
• Purity/Purification: Purified from cell culture supernatant by affinity chromatography; The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
• Form/Format: Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose is added as protectants before lyophilization.
• Sequence: CFI(Lys19-Val583) 6xHis tag

• Tag: C-6xHis tag
• Reconsitution: 1: Before opening the tube, centrifuge the sample tube at 5000g for 3-5 min at room temperature to ensure the lyophilized sample to settle down at the bottom of the tube.; 2: Dissolved lyophilized protein sample in water, cover the lid and mix them by gently tapping the tube for 5-10 times. Note : Do not vortex or vigorously pipette sample.
• Visual Inspection: Test: Lyophlization ; Specification: Dried white powder ; Result: Pass; Test: Vial Label ; Specification: Label aligned and complete; Results: Pass; Test: Bottle Sealing (manual check); Specification: Sealed tight; Results: Pass
• Quantity: Test : Bradford Method ; Specification: > specified quantity ; Results: Pass
• Other Properties: Test: pH ; Specification: 7.4 +/- 0.5; Results: Pass
• Preparation and Storage: Store at -20 degree C to -80 degree C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80 degree C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

SDS-PAGE

(Figure 1. Human CFI Protein, His Tag on SDS-PAGE under reducing condition.)

Related Product Information for CFI recombinant protein

Recombinant human CFI Protein with C-terminal 6xHis tag
This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene. [provided by RefSeq, Dec 2015]

Product Categories/Family for CFI recombinant protein

ECD 1182; ECD Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 119392081
• NCBI GeneID: 3426
• NCBI Accession #: NP_000195.2
• NCBI GenBank Nucleotide #: NM_000204.3
• UniProt Accession #: P05156; O60442
• Molecular Weight: The protein has a predicted molecular mass of 64.3 kDa after removal of the signal peptide. The apparent molecular mass of CFI-His is approximately 70-100 kDa due to glycosylation.
• NCBI Official Full Name: complement factor I preproprotein
• NCBI Official Synonym Full Names: complement factor I
• NCBI Official Symbol: CFI
• NCBI Official Synonym Symbols: FI; IF; KAF; AHUS3; C3BINA; C3b-INA
• NCBI Protein Information: complement factor I; C3b-inactivator; C3B/C4B inactivator; complement component I; light chain of factor I; Konglutinogen-activating factor; complement factor I heavy chain; complement control protein factor I
• UniProt Protein Name: Complement factor I
• UniProt Gene Name: CFI
• UniProt Synonym Gene Names: IF
• UniProt Entry Name: CFAI_HUMAN