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IF (Immunofluorescence)
(ICC/IF analysis of FABP7 in PC3 cells. The cell was stained with AAA11731 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Mouse anti-Human, Mouse FABP7 Monoclonal Antibody | anti-FABP7 antibody
FABP7 antibody
Gene Names
FABP7; MRG; BLBP; FABPB; B-FABP
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Synonyms
FABP7; Monoclonal Antibody; FABP7 antibody; Fatty acid binding protein 7; Fatty acid binding protein 7 B FABP; BFABP; BLBP; Brain lipid binding protein; DKFZp547J2313; FABP 7; FABPB; Fatty acid binding protein 7 brain; Fatty acid binding protein brain; OTTHuMP00000017119; Mammary derived growth inhibitor related; MRG.; anti-FABP7 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT1D1
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
132
Applicable Applications for anti-FABP7 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
Usage: The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Immunogen
Recombinant human FABP7 (1-132aa) purified from E. coli
Clone Note
Anti-human FABP7 mAb, clone AT1D1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human FABP7 protein.
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of FABP7 in PC3 cells. The cell was stained with AAA11731 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence)
(ICC/IF analysis of FABP7 in PC3 cells. The cell was stained with AAA11731 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunflorescense)
(ICC/IF analysis of FABP7 in U87MG cells. The cell was stained with AAA11731 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunflorescense)
(ICC/IF analysis of FABP7 in U87MG cells. The cell was stained with AAA11731 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
WB (Western Blot)
(The tissue lysate of mouse brain (60ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FABP7 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot)
(The tissue lysate of mouse brain (60ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FABP7 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot)
(The Mouse tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP7 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Testis tissue lysate)
WB (Western Blot)
(The Mouse tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP7 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Testis tissue lysate)
Related Product Information for anti-FABP7 antibody
FABP7 (Fatty acid binding protein 7) is also known as brain fatty acid-binding protein (B-FABP) and is a member of fatty acid-binding proteins (FABPs) which are a family of small, highly conserved, cytoplasmic proteins to bind long-chain fatty acids and other hydrophobic ligands. FABP7 is expressed in radial glia by the activation of Notch receptors and binds DHA with the highest affinity among all of FABPs.
Product Categories/Family for anti-FABP7 antibody
References
Borchers T, et al., (1997) Prostaglandins Leukot. Essent. Fatty Acids. 57 (1):77-84; Liu Rz, et al., (2003) Eur J Biochem. 270 (4):715-25
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,829 Da
NCBI Official Full Name
fatty acid-binding protein, brain isoform 1
NCBI Official Synonym Full Names
fatty acid binding protein 7
NCBI Official Symbol
FABP7
NCBI Official Synonym Symbols
MRG; BLBP; FABPB; B-FABP
NCBI Protein Information
fatty acid-binding protein, brain
UniProt Protein Name
Fatty acid-binding protein, brain
UniProt Gene Name
FABP7
UniProt Synonym Gene Names
BLBP; FABPB; MRG; BLBP; B-FABP
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Product Notes
The FABP7 fabp7 (Catalog #AAA11731) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FABP7 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's FABP7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). Usage: The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the FABP7 fabp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FABP7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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