Mouse CD314 Monoclonal Antibody | anti-CD314 antibody

MOUSE ANTI HUMAN CD314

Cat. Num. AAA11960

• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: CD314, Antibody; MOUSE ANTI HUMAN CD314; anti-CD314 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1D11
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 216

• Applicable Applications for anti-CD314 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Maximum Dilution: 1/100
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Immunogen: NKL cells.; Fusion Partners
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD314: Alexa Fluor 488 (AAA11960A488))

Application Data

(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . High power)

Application Data

(Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . high power)

Application Data

(Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)

Application Data

(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD314)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD314:Low Endotoxin)

Related Product Information for anti-CD314 antibody

Mouse anti Human CD314 antibody, clone 1D11 recognizes CD314, also known as natural killer receptor G2 (NKG2D) and as killer cell lectin-like receptor subfamily K, member 1 (KLRK1). CD314 is a C-type lectin-like activating receptor which is expressed on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. CD314 forms homodimers that signal through an associated DAP10 adaptor protein. Ligands of CD314 include MICA, MICB and UL16 binding protein (ULBP), which are inducibly expressed. Ligand binding toCD314 results in NK cell activation and potent costimulation of effector T cells. Clone 1D11 is reported to inhibit T cell recognition of MICA (Bauer et al. 1999).

NCBI and Uniprot Product Information

• NCBI GI #: 315221164
• NCBI GeneID: 100528032
• NCBI Accession #: NP_001186734.1
• NCBI GenBank Nucleotide #: NM_001199805.1
• UniProt Accession #: P26718; Q9NR41; A8K7K5; A8K7P4
• Molecular Weight: 25,274 Da
• NCBI Official Full Name: NKG2-D type II integral membrane protein
• NCBI Official Synonym Full Names: KLRC4-KLRK1 readthrough
• NCBI Official Symbol: KLRC4-KLRK1
• NCBI Protein Information: NKG2-D type II integral membrane protein; NK cell receptor D; NKG2-D-activating NK receptor; killer cell lectin-like receptor subfamily K member 1
• UniProt Protein Name: NKG2-D type II integral membrane protein
• UniProt Gene Name: KLRK1
• UniProt Synonym Gene Names: D12S2489E; NKG2D
• UniProt Entry Name: NKG2D_HUMAN