3-Methoxytyramine (3-MT) ELISA Kit | 3-MT elisa kit
General species 3-Methoxytyramine (3-MT)(Competitive EIA) ELISA Kit
Reactivity
General species
Applications
ELISA
Synonyms
3-Methoxytyramine (3-MT); N/A; General species 3-Methoxytyramine (3-MT)(Competitive EIA) ELISA Kit; ELISA Kit for 3-Methoxytyramine (3-MT); 3-MT/3-Methoxytyramine; 3-Methoxy-4-Hydroxyphenethylamine; 3-MT elisa kit
Reactivity
General species
Specificity
This assay has high sensitivity and excellent specificity for detection of 3-MT.
Assay Type
Quantitative Competitive
Samples
Serum, plasma and other biological fluids
Detection Range
61.7-5,000pg/mL
Sensitivity
< 21.9pg/mL
Applicable Applications for 3-MT elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 3-MT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 3-MT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 4 degree C for 1 month/ -20 degree C for long-term (within shelf life).
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for 3-MT elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 3-MT has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 3-MT and unlabeled 3-MT (Standards or samples) with the pre-coated antibody specific to 3-MT. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 3-MT in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 3-MT in the sample.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of 3-MT in serum, plasma and other biological fluids.
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of 3-MT in serum, plasma and other biological fluids.
