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Application Data
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Mouse MHC CLASS II RT1B Monoclonal Antibody

MOUSE ANTI RAT MHC CLASS II RT1B

Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
MHC CLASS II RT1B, Antibody; MOUSE ANTI RAT MHC CLASS II RT1B; anti-MHC CLASS II RT1B antibody
Ordering
 
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For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
OX-6
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Applicable Applications for anti-MHC CLASS II RT1B antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunohistology Paraffin*, Western Blot (WB)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Immunohistology - Paraffin: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results.
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Immunogen
Rat thymocyte membrane glycoprotein
Fusion Partners
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Application Data

(Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B)

Application Data

(Staining of rat spleen cells with Mouse anti Rat RT1B)

Application Data

(Staining of rat spleen cells with Mouse anti Rat RT1B: FITC)

Application Data

(Staining of rat spleen cells with Mouse anti Rat RT1B: RPE)

Application Data

(Staining of rat spleen cells with Mouse anti Rat RT1B: FITC)

Application Data

(Rat Spleen Tissue lysate probed with Mouse anti Rat MHC Class II RT1B)

Application Data

(Staining of rat spleen cells with Mouse anti Rat RTIB: Alexa Fluor 647)

Application Data

(Staining of rat spleen cells with Mouse anti Rat RT1B: Alexa Fluor 488)

Related Product Information for anti-MHC CLASS II RT1B antibody
Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 recognizes a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Rat MHC Class II RT1B antibody, clone OX-6 does not react with the rat BDIX strain due to a defect in RT1B expression (Male et al. 1987). The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region. Mouse anti Rat MHC Class II RT1B antibody, clone OX-6 also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region (McMaster and Williams 1979 and Male et al. 1987). This product is routinely tested in flow cytometry on rat splenocytes.

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Product Notes

The MHC CLASS II RT1B (Catalog #AAA11975) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MHC CLASS II RT1B can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunohistology Paraffin*, Western Blot (WB). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100 Immunohistology - Paraffin: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results. Researchers should empirically determine the suitability of the MHC CLASS II RT1B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC CLASS II RT1B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.