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FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with Apolipoprotein A II antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human, Rat Apolipoprotein A II Monoclonal Antibody | anti-APOA2 antibody
Apolipoprotein A II Antibody
Gene Names
APOA2; apoAII; Apo-AII; ApoA-II
Reactivity
Human, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Apolipoprotein A II; Monoclonal Antibody; Apolipoprotein A II Antibody; APO A2 antibody; Apo AII antibody; Apo-AII antibody; APOA 2 antibody; ApoA II antibody; ApoA-II antibody; APOA2 antibody; APOA2_HUMAN antibody; APOAII antibody; Apolipoprotein A II antibody; Apolipoprotein A-II (1-76) antibody; Apolipoprotein A2 antibody; Apolipoprotein AII antibody; ApolipoproteinA II antibody; OTTHUMP00000032244 antibody; anti-APOA2 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Monoclonal
Clone Number
JB70-35
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-APOA2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with Apolipoprotein A II antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with Apolipoprotein A II antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining Apolipoprotein A II in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Apolipoprotein A II in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Apolipoprotein A II in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Apolipoprotein A II in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Apolipoprotein A II in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Apolipoprotein A II in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat big intestine tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat big intestine tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.)
Related Product Information for anti-APOA2 antibody
s are protein components of plasma lipoproteins. The human apoA-I gene encodes a single chain, 243 amino acid protein which promotes cholesterol efflux from tissues to the liver for excretion. Apolipoprotein A-I is the major protein component of high density lipoprotein (HDL) in the plasma. It can function as a co-factor for lecithin cholesterolacyltransferase (LCAT), which is responsible for the formation of most plasma cholesterylesters. The human apoA-II gene encodes the second most abundant protein of HDL particles, where it influences plasma levels of free fatty acids (FFA). The human apoA-IV gene encodes a 396 amino acid preprotein, which after proteolytic processing is secreted from the intestine in association with chylomicron particles. ApoA-IV is a potent activator of LCAT in vitro. The human apoA-V gene encodes a 366 amino acid protein that is believed to be an important determinant of plasma triglyceride levels.
Product Categories/Family for anti-APOA2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,175 Da
NCBI Official Full Name
apolipoprotein A-II preproprotein
NCBI Official Synonym Full Names
apolipoprotein A-II
NCBI Official Symbol
APOA2
NCBI Official Synonym Symbols
apoAII; Apo-AII; ApoA-II
NCBI Protein Information
apolipoprotein A-II; apolipoprotein A2
UniProt Protein Name
Apolipoprotein A-II
UniProt Gene Name
APOA2
UniProt Synonym Gene Names
Apo-AII; ApoA-II
UniProt Entry Name
APOA2_HUMAN
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Product Notes
The APOA2 apoa2 (Catalog #AAA30454) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Apolipoprotein An II Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Apolipoprotein An II can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500 IHC: 1:50-1:200 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the APOA2 apoa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apolipoprotein A II, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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