Rat CD206 Monoclonal Antibody | anti-CD206 antibody
RAT ANTI MOUSE CD206:RPE
Flow Cytometry: Maximum Dilution: Neat; Application Note: CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. We recommends the use of Leucoperm for this purpose.
For better results an overnight incubation at 4 degree C is recommended
Perservative Stabilisers
Shelf Life: 12 months from date of reconstitution.
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
Application Data
(Published customer image: Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1beta. (A) Representative western blotting data of primary microglial cells produced from wild-type (wild) and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1beta. Each lane expected to CD206 blotting were applied 7 ug of reduced samples. Non-reduced samples (5 ug) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1beta and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1beta and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1beta. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1beta. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1beta; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1beta for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65.)
Application Data
(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. Low power)
Application Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)
Application Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)
Application Data
(Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm)
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 488)
Application Data
(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power)
Application Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)
Application Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 647)
