WB (Western Blot)
(Detection of human CD206/Mannose Receptor by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MUTZ-3, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody .)
CD206/Mannose Receptor Recombinant Antibody | CD206 recombinant antibody
Rabbit anti-CD206/Mannose Receptor Recombinant Monoclonal Antibody [BLR109H]
IP: 6 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD206/Mannose Receptor by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MUTZ-3, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human CD206/Mannose Receptor by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MUTZ-3, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human CD206/Mannose Receptor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MUTZ-3 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1) used for IP at 6 ul per reaction. CD206/Mannose Receptor was also immunoprecipitated by rabbit anti-CD206/Mannose Receptor antibody 1507A-3G8. For blotting immunoprecipitated CD206/Mannose Receptor, AAA23836 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human CD206/Mannose Receptor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MUTZ-3 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1) used for IP at 6 ul per reaction. CD206/Mannose Receptor was also immunoprecipitated by rabbit anti-CD206/Mannose Receptor antibody 1507A-3G8. For blotting immunoprecipitated CD206/Mannose Receptor, AAA23836 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human CD206/Mannose Receptor in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human CD206/Mannose Receptor in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human CD206/Mannose Receptor in FFPE non-Hodgkin's lymphoma by IHC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human CD206/Mannose Receptor in FFPE non-Hodgkin's lymphoma by IHC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human CD206/Mannose Receptor in FFPE lung carcinoma pleural effusion by IHC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human CD206/Mannose Receptor in FFPE lung carcinoma pleural effusion by IHC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CD206/Mannose Receptor in FFPE MUTZ-3 cells by ICC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human CD206/Mannose Receptor in FFPE MUTZ-3 cells by ICC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
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Product Notes
The CD206 mrc1 (Catalog #AAA23836) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD206/Mannose Receptor Recombinant Monoclonal Antibody [BLR109H] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD206/Mannose Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC). WB: 1:1000 IP: 6 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Researchers should empirically determine the suitability of the CD206 mrc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD206/Mannose Receptor, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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