Mouse CD45 Monoclonal Antibody | anti-CD45 antibody

MOUSE ANTI HUMAN CD45:FITC

Cat. Num. AAA12202

• Gene Names: PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD45, Antibody; MOUSE ANTI HUMAN CD45:FITC; anti-CD45 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: F10-89-4
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 1306

• Applicable Applications for anti-CD45 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood. ; Flow Cytometry: Maximum Dilution: Neat
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Serum Albumin; Preparation
• Immunogen: Human T lymphocytes; Fusion Partners
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD45: Pacific Blue)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:PECy5.5)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:Biotin)

Application Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD45:Alexa Fluor 647)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:APC)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti HumanCD45:Alexa Fluor 488)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:RPE)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:FITC)

Related Product Information for anti-CD45 antibody

Mouse anti Human CD45 antibody, clone F10-89-4 recognizes the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc. Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. Mouse anti Human CD45 antibody, clone F10-89-4 is routinely tested in flow cytometry on human peripheral blood leucocytes

NCBI and Uniprot Product Information

• NCBI GI #: 392307007
• NCBI GeneID: 5788
• NCBI Accession #: NP_002829.3
• NCBI GenBank Nucleotide #: NM_002838.4
• UniProt Accession #: P08575; Q16614; Q9H0Y6; A8K7W6
• Molecular Weight: 130,898 Da
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase C isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type, C
• NCBI Official Symbol: PTPRC
• NCBI Official Synonym Symbols: LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• UniProt Gene Name: PTPRC
• UniProt Synonym Gene Names: CD45; L-CA
• UniProt Entry Name: PTPRC_HUMAN