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Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD45: Pacific Blue)
Mouse CD45 Monoclonal Antibody | anti-CD45 antibody
MOUSE ANTI HUMAN CD45:APC
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Applications
Flow Cytometry, Functional Assay
Synonyms
CD45; Monoclonal Antibody; MOUSE ANTI HUMAN CD45:APC; anti-CD45 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
F10-89-4
Form/Format
APC
Purified IgG conjugated to Allophycocyanin (APC) - lyophilized
Purified IgG conjugated to Allophycocyanin (APC) - lyophilized
Sequence Length
1306
Applicable Applications for anti-CD45 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood
Flow Cytometry: Maximum Dilution: Neat
Flow Cytometry: Maximum Dilution: Neat
Reconstitution
Reconstitute with 1 ml distilled water
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
1% Bovine Serum Albumin
5% Sucrose
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Immunogen
Human T lymphocytes
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Buffer Solution
Phosphate buffered saline
Target Species
Human
RRID
AB_324884
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Shelf Life: 12 months from date of reconstitution.
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD45: Pacific Blue)
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD45: Pacific Blue)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:PECy5.5)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:PECy5.5)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:Biotin)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:Biotin)
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD45:Alexa Fluor 647)
Application Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD45:Alexa Fluor 647)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:APC)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:APC)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti HumanCD45:Alexa Fluor 488)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti HumanCD45:Alexa Fluor 488)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:RPE)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:RPE)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:FITC)
Application Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD45:FITC)
Related Product Information for anti-CD45 antibody
Mouse anti Human CD45 antibody, clone F10-89-4 recognizes the human CD45 cell surface antigen, also known as the leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc. Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. Mouse anti Human CD45 antibody, clone F10-89-4 is routinely tested in flow cytometry on human peripheral blood leucocytes
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
130,898 Da
NCBI Official Full Name
receptor-type tyrosine-protein phosphatase C isoform 1
NCBI Official Synonym Full Names
protein tyrosine phosphatase, receptor type, C
NCBI Official Symbol
PTPRC
NCBI Official Synonym Symbols
LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
NCBI Protein Information
receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide
UniProt Protein Name
Receptor-type tyrosine-protein phosphatase C
UniProt Gene Name
PTPRC
UniProt Synonym Gene Names
CD45; L-CA
UniProt Entry Name
PTPRC_HUMAN
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Product Notes
The CD45 ptprc (Catalog #AAA11843) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD45 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD45 ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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