Mouse CD4 Monoclonal Antibody | anti-CD4 antibody

MOUSE ANTI HUMAN CD4

Cat. Num. AAA12209

• Gene Names: CD4; CD4mut
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: CD4, Antibody; MOUSE ANTI HUMAN CD4; anti-CD4 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: RPA-T4
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1mg/ml (varies by lot)
• Sequence Length: 458

• Applicable Applications for anti-CD4 antibody: Immunohistology Frozen*, Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood ; Immunohistology - Frozen: Minimum Dilution: 1/10; Maximum Dilution: 1/100; Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Histology Positive Control Tissue: Human lymphatic tissue; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Pacific Blue)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4: Alexa Fluor 488)

Application Data

(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Low power)

Application Data

(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. Medium power)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:RPE)

Application Data

(Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD4 antibody, clone RPA-T4 followed by Histar Detection System. High power)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:Biotin)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:FITC)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4:Alexa Fluor 405)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD4)

NCBI and Uniprot Product Information

• NCBI GI #: 10835167
• NCBI GeneID: 920
• NCBI Accession #: NP_000607.1
• NCBI GenBank Nucleotide #: NM_000616.4
• Molecular Weight: 51,111 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4 isoform 1
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 antigen (p55); CD4 receptor; T-cell surface antigen T4/Leu-3
• UniProt Protein Name: T-cell surface glycoprotein CD4
• UniProt Gene Name: CD4
• UniProt Entry Name: CD4_HUMAN