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ELISA
(Detection limit for recombinant GST tagged DARS is approximately 0.3 ng/ml as a capture antibody.)
Mouse anti-Human DARS Monoclonal Antibody | anti-DARS antibody
Mouse Monoclonal [clone 2F11] (IgG2a,k) to Human DARS
Gene Names
DARS; HBSL; aspRS
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
DARS; Monoclonal Antibody; Mouse Monoclonal [clone 2F11] (IgG2a; k) to Human DARS; Anti-DARS Antibody (clone 2F11) IHC-plus; Aspartyl-tRNA synthetase; AspRS; Human DARS; anti-DARS antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2F11
Purity/Purification
Protein A Purified
Form/Format
PBS, pH 7.2. Sourced in Ascites.
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
501
Applicable Applications for anti-DARS antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA), RNA interference
Application Notes
ELISA, IHC-P (5 ug/ml), RNAi, WB
Usage: Western Blot (Cell lysate) - positive control HeLa. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated).
Usage: Western Blot (Cell lysate) - positive control HeLa. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated).
Target Species
Human
Immunogen Description
DARS (NP_001340, 393 a.a. ~ 501 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
DARS antibody was raised against dARS (NP_001340, 393 a.a. ~ 501 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
ELISA
(Detection limit for recombinant GST tagged DARS is approximately 0.3 ng/ml as a capture antibody.)
ELISA
(Detection limit for recombinant GST tagged DARS is approximately 0.3 ng/ml as a capture antibody.)
RNA interference
(Western blot of DARS over-expressed 293 cell line, cotransfected with DARS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DARS monoclonal antibody, clone 2F11. GAPDH (36.1 kD) used as specificity and.)
RNA interference
(Western blot of DARS over-expressed 293 cell line, cotransfected with DARS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DARS monoclonal antibody, clone 2F11. GAPDH (36.1 kD) used as specificity and.)
WB (Western Blot)
(DARS monoclonal antibody, clone 2F11 Western blot of DARS expression in HeLa.)
WB (Western Blot)
(DARS monoclonal antibody, clone 2F11 Western blot of DARS expression in HeLa.)
WB (Western Blot)
(Western blot of DARS expression in transfected 293T cell line by DARS monoclonal antibody, clone 2F11.)
WB (Western Blot)
(Western blot of DARS expression in transfected 293T cell line by DARS monoclonal antibody, clone 2F11.)
IHC (Immunohistochemistry)
(Anti-DARS antibody IHC of human ganglion cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-DARS antibody IHC of human ganglion cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-DARS antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-DARS antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-DARS antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
IHC (Immunohistochemistry)
(Anti-DARS antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Related Product Information for anti-DARS antibody
Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,771 Da
NCBI Official Full Name
aspartate--tRNA ligase, cytoplasmic isoform 1
NCBI Official Synonym Full Names
aspartyl-tRNA synthetase
NCBI Official Symbol
DARS
NCBI Official Synonym Symbols
HBSL; aspRS
NCBI Protein Information
aspartate--tRNA ligase, cytoplasmic; aspartate tRNA ligase 1, cytoplasmic; aspartyl-tRNA synthetase, cytoplasmic; cell proliferation-inducing gene 40 protein
UniProt Protein Name
Aspartate--tRNA ligase, cytoplasmic
UniProt Gene Name
DARS
UniProt Synonym Gene Names
AspRS
UniProt Entry Name
SYDC_HUMAN
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Product Notes
The DARS dars (Catalog #AAA12350) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 2F11] (IgG2a,k) to Human DARS reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DARS can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA), RNA interference. ELISA, IHC-P (5 ug/ml), RNAi, WB Usage: Western Blot (Cell lysate) - positive control HeLa. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein). RNAi Knockdown (Antibody validated). Researchers should empirically determine the suitability of the DARS dars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DARS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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