Reactivity
Human
Specificity
No cross-reaction with other factors.
Sequence Length
543
Assay Type
Sandwich
Detection Range
2000 pg/mL-31.2 pg/mL
Sensitivity
Up to 12 pg/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for HPA elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human HPA monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
42,791 Da
NCBI Official Full Name
heparanase
NCBI Official Synonym Full Names
heparanase
NCBI Official Symbol
HPSE
NCBI Official Synonym Symbols
HPA; HPA1; HPR1; HSE1; HPSE1
NCBI Protein Information
heparanase; heparanase-1; endo-glucoronidase
UniProt Protein Name
Heparanase
UniProt Gene Name
HPSE
UniProt Synonym Gene Names
HEP; HPA; HPA1; HPR1; HPSE1; HSE1; Hpa1
UniProt Entry Name
HPSE_HUMAN