Mouse IgG ELISA Kit | IgG elisa kit
Mouse IgG (Total) ELISA Kit
Reactivity
Mouse
Synonyms
IgG; N/A; Mouse IgG (Total) ELISA Kit; IgG elisa kit
Reactivity
Mouse
Specificity
This assay recognizes both recombinant and natural mouse IgG. The factors listed below were prepared at 50ng/ml and assayed for cross-reactivity.
Sequence Length
476
Assay Type
Quantitative Sandwich
Detection Range
1.56-100ng/mL
Sensitivity
< 0.08ng/mL
Intra-assay Precision
Intra-plate Precision The three samples with known concentrations were repeated 20 times on the same board to calculate the coefficient of variation (CV) for the concentrations. Intra-Assay: CV<10%
Inter-assay Precision
Inter-plate Precision The coefficient of variation (CV) of three known concentrations was calculated by 20 tests of two kits on each of the three samples. Inter-Assay: CV<15%
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Related Product Information for IgG elisa kit
Background: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IgG in mouse serum, plasma, cell culture supernatants, cell lysates, tissue homogenates and other biological fluids.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and enzyme conjugate detection antibody is added to the wells. Following incubation and wash steps, a substrate solution is added to the wells and color develops in proportion to the amount of IgG bound in the initial step. The color development is stopped and the absorbance is measured.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and enzyme conjugate detection antibody is added to the wells. Following incubation and wash steps, a substrate solution is added to the wells and color develops in proportion to the amount of IgG bound in the initial step. The color development is stopped and the absorbance is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
IgG
