Mouse Myosin Light Chain Kinase (MLCK) ELISA Kit | MLCK elisa kit
Mouse Myosin Light Chain Kinase (MLCK) ELISA Kit
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Reactivity
Mouse
Synonyms
Myosin Light Chain Kinase (MLCK); N/A; Mouse Myosin Light Chain Kinase (MLCK) ELISA Kit; MLCK elisa kit
Reactivity
Mouse
Sequence Length
1914
Assay Type
Sandwich
Detection Range
20 ng/mL-0.312 ng/mL
Sensitivity
Up to 0.06 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for MLCK elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Mouse MLCK monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
110,076 Da
NCBI Official Full Name
myosin light chain kinase
NCBI Official Synonym Full Names
myosin light chain kinase
NCBI Official Symbol
MYLK
NCBI Official Synonym Symbols
KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
NCBI Protein Information
myosin light chain kinase, smooth muscle; telokin; kinase-related protein; myosin, light polypeptide kinase; smooth muscle myosin light chain kinase
UniProt Protein Name
Myosin light chain kinase, smooth muscle
UniProt Gene Name
MYLK
UniProt Synonym Gene Names
MLCK; MLCK1; MYLK1; MLCK; smMLCK; KRP
UniProt Entry Name
MYLK_HUMAN