Monkey Cardiac myosin-light chains 1 ELISA Kit | CMIC-1 elisa kit

Monkey Cardiac myosin-light chains 1 ELISA Kit

Cat. Num. AAA16845

• Gene Names: cmlc1; zgc:103441
• Reactivity: Monkey
• Synonyms: Cardiac myosin-light chains 1; N/A; Monkey Cardiac myosin-light chains 1 ELISA Kit; CMIC-1 elisa kit

• Reactivity: Monkey
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 5ug/mL
• Sensitivity: 0.1ug/mL

• Intended Uses: This CM-Lc1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CM-Lc1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CMIC-1 elisa kit

Principle of the assay: CM-Lc1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CM-Lc1 antibody and an CM-Lc1-HRP conjugate. The assay sample and buffer are incubated together with CM-Lc1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CM-Lc1 concentration since CM-Lc1 from samples and CM-Lc1-HRP conjugate compete for the anti-CM-Lc1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CM-Lc1 from the sample, fewer sites are left to bind CM-Lc1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CM-Lc1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CMIC-1 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 55926111
• NCBI GeneID: 64671
• NCBI Accession #: NP_571767.1
• NCBI GenBank Nucleotide #: NM_131692.1
• NCBI Official Full Name: cardiac myosin light chain-1
• NCBI Official Synonym Full Names: cardiac myosin light chain-1
• NCBI Official Symbol: cmlc1
• NCBI Official Synonym Symbols: zgc:103441
• NCBI Protein Information: cardiac myosin light chain-1; laz; lazy susan