Mouse anti-Bullous Pemphigoid 180 antibody (BP180-Ab) ELISA Kit | BP180-Ab elisa kit
Mouse anti-Bullous Pemphigoid 180 antibody (BP180-Ab) ELISA Kit
Reactivity
Mouse
Synonyms
anti-Bullous Pemphigoid 180 antibody (BP180-Ab); N/A; Mouse anti-Bullous Pemphigoid 180 antibody (BP180-Ab) ELISA Kit; BP180-Ab elisa kit
Reactivity
Mouse
Specificity
No cross-reaction with other factors.
Assay Type
Sandwich
Samples
Mouse serum, plasma, or cell culture supernatant
Detection Range
20 ng/mL-0.312 ng/mL
Sensitivity
Up to 0.06 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for BP180-Ab elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antigen Sandwich ELISA technique. The pre-coated antigen is a related-Mouse BP180-Ab antigen, while the detection antigen is another antigen. Samples are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
