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Standard Curve (Sample)

Human Anti Low-density lipoprotein receptor-related protein 4 antibody (LRP-4-Ab) ELISA Kit | LRP-4-Ab elisa kit

Human Anti Low-density lipoprotein receptor-related protein 4 antibody (LRP-4-Ab) ELISA Kit

Gene Names
Lrp4; mdig; Megf7; D230026E03; 6430526J12Rik
Reactivity
Human
Synonyms
Anti Low-density lipoprotein receptor-related protein 4 antibody (LRP-4-Ab); Human Anti Low-density lipoprotein receptor-related protein 4 antibody (LRP-4-Ab) ELISA Kit; LRP-4-Ab elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of LRP4Ab. No significant cross-reactivity or interference between LRP4Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LRP4Ab and all the analogues, therefore, cross reaction may still exist in some cases.
Sequence Length
519
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 ng/mL

Standard Curve (Sample)

Standard Curve (Sample)
Related Product Information for LRP-4-Ab elisa kit
Principle of the Assay: LRP4Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Low-density lipoprotein receptor-related protein 4 antigen and an LRP4Ab-HRP conjugate. The assay sample and buffer are incubated together with LRP4Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LRP4Ab concentration since LRP4Ab from samples and LRP4Ab-HRP conjugate compete for the Low-density lipoprotein receptor-related protein 4 antigen binding site. Since the number of sites is limited, as more sites are occupied by LRP4Ab from the sample, fewer sites are left to bind LRP4Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LRP4Ab concentration in each sample is interpolated from this standard curve.

Intended Uses: This LRP4Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LRP4Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
205,373 Da
NCBI Official Full Name
low-density lipoprotein receptor-related protein 4 isoform 2
NCBI Official Synonym Full Names
low density lipoprotein receptor-related protein 4
NCBI Official Symbol
Lrp4
NCBI Official Synonym Symbols
mdig; Megf7; D230026E03; 6430526J12Rik
NCBI Protein Information
low-density lipoprotein receptor-related protein 4
UniProt Protein Name
Low-density lipoprotein receptor-related protein 4
UniProt Gene Name
Lrp4
UniProt Synonym Gene Names
Kiaa0816; LRP-4

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Product Notes

The Human LRP-4-Ab lrp4 (Catalog #AAA27394) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA27394 ELISA Kit recognizes Human LRP-4-Ab. It is sometimes possible for the material contained within the vial of "Anti Low-density lipoprotein receptor-related protein 4 antibody (LRP-4-Ab), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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