Porcine Surfactant Associated Protein D (SPD) ELISA Kit | SPD elisa kit
Porcine Surfactant Associated Protein D (SPD)
Gene Names
Sftpd; SP-D; Sftp4; AI573415
Reactivity
Porcine
Synonyms
Surfactant Associated Protein D (SPD); N/A; Porcine Surfactant Associated Protein D (SPD); SPD elisa kit
Reactivity
Porcine
Sequence Length
374
Assay Type
Sandwich
Detection Range
100 ng/mL-1.56 ng/mL
Sensitivity
Up to 0.5 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for SPD elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Porcine SPD monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
37,688 Da
NCBI Official Full Name
Surfactant associated protein D
NCBI Official Synonym Full Names
surfactant associated protein D
NCBI Official Symbol
Sftpd
NCBI Official Synonym Symbols
SP-D; Sftp4; AI573415
NCBI Protein Information
pulmonary surfactant-associated protein D; PSP-D; lung surfactant protein D
UniProt Protein Name
Pulmonary surfactant-associated protein D
UniProt Gene Name
Sftpd
UniProt Synonym Gene Names
Sftp4; PSP-D; SP-D
UniProt Entry Name
SFTPD_MOUSE
