Human Pulmonary Surfactant Associated Protein D ELISA Kit | SPD elisa kit
Human Pulmonary Surfactant Associated Protein D ELISA Kit
Gene Names
MBL2; MBL1; cMBl; collectin
Reactivity
Human
Synonyms
Pulmonary Surfactant Associated Protein D; N/A; Human Pulmonary Surfactant Associated Protein D ELISA Kit; SPD (Pulmonary Surfactant Associated Protein D)/SFTPD/SP-D/Collectin 7/PSP-D/SFTP4/COLEC7/collectin-7/Lung surfactant protein D/PSPD/pulmonary surfactant-associated protein D/pulmonary surfactant apoprotein/surfactant protein D/surfactant; pulmonary-associated protein D/surfactant-associated protein; pulmonary 4; SPD elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of SPD. No significant cross-reactivity or interference between SPD and analogues was observed.
Sequence Length
254
Assay Type
Sandwich
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
1.563-100ng/ml
Sensitivity
0.938ng/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Related Product Information for SPD elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
pulmonary surfactant-associated protein D
NCBI Official Synonym Full Names
mannose-binding lectin (protein C) 2, soluble
NCBI Official Symbol
MBL2
NCBI Official Synonym Symbols
MBL1; cMBl; collectin
NCBI Protein Information
pulmonary surfactant-associated protein D