Chicken Aspartate aminotransferase (AST) ELISA Kit | AST elisa kit

Chicken Aspartate aminotransferase (AST) ELISA Kit

Cat. Num. AAA12753

• Gene Names: ASP5; AAT3; ASP5; ASPARTATE AMINOTRANSFERASE; aspartate aminotransferase 5; ASPARTATE AMINOTRANSFERASE DEFICIENT 3; ATAAT1; F11C18.15
• Reactivity: Chicken
• Synonyms: Aspartate aminotransferase (AST); N/A; Chicken Aspartate aminotransferase (AST) ELISA Kit; AST elisa kit

• Reactivity: Chicken
• Sequence Length: 448
• Assay Type: Sandwich
• Detection Range: 20 ng/mL-0.312 ng/mL
• Sensitivity: Up to 0.06 ng/mL

• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for AST elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Chicken AST monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 334187077
• NCBI GeneID: 829330
• NCBI Accession #: NP_001190885.1
• NCBI GenBank Nucleotide #: NM_001203956.1
• Molecular Weight: 49,831 Da
• NCBI Official Full Name: aspartate aminotransferase
• NCBI Official Symbol: ASP5
• NCBI Official Synonym Symbols: AAT3; ASP5; ASPARTATE AMINOTRANSFERASE; aspartate aminotransferase 5; ASPARTATE AMINOTRANSFERASE DEFICIENT 3; ATAAT1; F11C18.15
• NCBI Protein Information: aspartate aminotransferase
• UniProt Protein Name: Aspartate aminotransferase, chloroplastic
• UniProt Gene Name: ASP5
• UniProt Synonym Gene Names: AAT1; At4g31990; F10N7.200
• UniProt Entry Name: AAT5_ARATH