Aspartate Aminotransferase(AST) Assay Kit | AST assay kit
Aspartate Aminotransferase(AST) assay kit
Synonyms
Aspartate Aminotransferase(AST); N/A; Aspartate Aminotransferase(AST) assay kit; AST assay kit
Sequence Length
393
Samples
Animal serum (plasma), tissue, culture cells and cell culture supernatant, etc
Detection Range
1.1-72.3 IU/L
Sensitivity
1.1 IU/L
Intra-assay Precision
The intra-assay CV is 5.3%.
Inter-assay Precision
The inter-assay CV is 6.8%.
Preparation and Storage
Store at 2-8 degree C. Valid for 12 months
Standard Curve (Sample)
Related Product Information for AST assay kit
Principle of the Assay: AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4-dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
aspartate aminotransferase
