Reactivity
Goat
Sequence Length
307
Assay Type
Sandwich
Detection Range
20 ng/mL-0.312 ng/mL
Sensitivity
Up to 0.06 ng/mL
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for HS elisa kit
Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Goat HS monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,773 Da
NCBI Official Full Name
heparan sulfate glucosamine 3-O-sulfotransferase 1
NCBI Official Synonym Full Names
heparan sulfate (glucosamine) 3-O-sulfotransferase 1
NCBI Official Symbol
HS3ST1
NCBI Official Synonym Symbols
3OST; 3OST1
NCBI Protein Information
heparan sulfate glucosamine 3-O-sulfotransferase 1; 3-OST-1; h3-OST-1; heparan sulfate 3-O-sulfotransferase 1; heparin-glucosamine 3-O-sulfotransferase; heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1
UniProt Protein Name
Heparan sulfate glucosamine 3-O-sulfotransferase 1
UniProt Gene Name
HS3ST1
UniProt Synonym Gene Names
3OST; 3OST1; 3-OST-1; Heparan sulfate 3-O-sulfotransferase 1; h3-OST-1
UniProt Entry Name
HS3S1_HUMAN