Fish Estradiol ELISA Kit | E2 elisa kit
Fish Estradiol (E2) ELISA Kit
Reactivity
Fish
Synonyms
Estradiol; N/A; Fish Estradiol (E2) ELISA Kit; E2 elisa kit
Reactivity
Fish
Specificity
This assay has high sensitivity and excellent specificity for detection of Fish E2. No significant cross-reactivity or interference between Fish E2 and analogues was observed.
Assay Type
Quantitative Competitive
Samples
Serum, plasma and other biological fluids
Detection Range
50 pg/mL - 1200 pg/mL
Sensitivity
20 pg/mL (mean of 6 independent assays)
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for E2 elisa kit
Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for E2 and Horseradish Peroxidase (HRP) conjugated E2. The competitive inhibition reaction is launched between with HRP labeled E2 and unlabeled E2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of E2 in the sample. The color development is stopped and the intensity of the color is measured.
Intended Uses: For the quantitative detection of Fish Estradiol (E2) concentration in serum, plasma and other biological fluids.
Intended Uses: For the quantitative detection of Fish Estradiol (E2) concentration in serum, plasma and other biological fluids.