Rat E2 estradiol ELISA Kit | E2 elisa kit
Rat E2 estradiol ELISA Kit
Reactivity
Rat
Synonyms
E2 estradiol; N/A; Rat E2 estradiol ELISA Kit; E2 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of E2. No significant cross-reactivity or interference between E2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between E2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for E2 elisa kit
Intended Uses: This E2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat E2. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: E2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-E2 antibody and an E2-HRP conjugate. The assay sample and buffer are incubated together with E2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the E2 concentration since E2 from samples and E2-HRP conjugate compete for the anti-E2 antibody binding site. Since the number of sites is limited, as more sites are occupied by E2 from the sample, fewer sites are left to bind E2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The E2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: E2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-E2 antibody and an E2-HRP conjugate. The assay sample and buffer are incubated together with E2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the E2 concentration since E2 from samples and E2-HRP conjugate compete for the anti-E2 antibody binding site. Since the number of sites is limited, as more sites are occupied by E2 from the sample, fewer sites are left to bind E2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The E2 concentration in each sample is interpolated from this standard curve.
References
Effects of Pine Pollen Extract in Relieving Hot Flushes in Sex Hormone-Deficienct Rats. Panida Chamawan, Krittiya Thisayakorn, Srichan Phornchirasilp (2017)
