Mouse High mobility group protein B1 (HMGB1) ELISA Kit | HMGB1 elisa kit
Mouse High mobility group protein B1 (HMGB1) ELISA Kit
Gene Names
Hmgb1; p30; Hmg1; HMG-1; SBP-1
Reactivity
Mouse
Synonyms
High mobility group protein B1 (HMGB1); N/A; Mouse High mobility group protein B1 (HMGB1) ELISA Kit; DKFZp686A04236; HMG1; HMG3; SBP-1; Amphoterin; OTTHUMP00000018196; OTTHUMP00000190860; OTTHUMP00000200117; Sulfoglucuronyl carbohydrate binding protein; high mobility group box 1; high mobility group prot; HMGB1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse HMGB-1. No significant cross-reactivity or interference between Mouse HMGB-1 and analogues was observed.
Sequence Length
215
Assay Type
Quantitative Sandwich
Samples
Serum, plasma and other biological fluids
Detection Range
7.8 pg/mL - 500 pg/mL
Sensitivity
5.2 pg/mL (mean of 6 independent assays)
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for HMGB1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HMGB-1 in Mouse serum, plasma. An antibody specific for HMGB-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HMGB-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HMGB-1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HMGB-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Intended Uses: For the quantitative detection of Mouse High mobility group protein B1 (HMGB-1) concentration in serum, plasma and other biological fluids.
Intended Uses: For the quantitative detection of Mouse High mobility group protein B1 (HMGB-1) concentration in serum, plasma and other biological fluids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,894 Da
NCBI Official Full Name
high mobility group protein B1
NCBI Official Synonym Full Names
high mobility group box 1
NCBI Official Symbol
Hmgb1
NCBI Official Synonym Symbols
p30; Hmg1; HMG-1; SBP-1
NCBI Protein Information
high mobility group protein B1
UniProt Protein Name
High mobility group protein B1
UniProt Gene Name
Hmgb1
UniProt Synonym Gene Names
Hmg-1; Hmg1; HMG-1