Mouse High Mobility Group box1 Protein ELISA Kit | HMGB1 elisa kit
Mouse High Mobility Group box1 Protein ELISA Kit
Gene Names
HMGB1; HMG1; HMG3; SBP-1
Reactivity
Mouse
Synonyms
High Mobility Group box1 Protein; N/A; Mouse High Mobility Group box1 Protein ELISA Kit; HMGB1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of HMGB1. No significant cross-reactivity or interference between HMGB1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HMGB1 and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for HMGB1 elisa kit
Principle of the Assay: HMGB1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HMGB1 antibody and an HMGB1-HRP conjugate. The assay sample and buffer are incubated together with HMGB1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HMGB1 concentration since HMGB1 from samples and HMGB1-HRP conjugate compete for the anti-HMGB1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HMGB1 from the sample, fewer sites are left to bind HMGB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMGB1 concentration in each sample is interpolated from this standard curve.
Intended Uses: This HMGB1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HMGB1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This HMGB1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HMGB1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for HMGB1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
24,894 Da
NCBI Official Full Name
HMGB1
NCBI Official Synonym Full Names
high mobility group box 1
NCBI Official Symbol
HMGB1
NCBI Official Synonym Symbols
HMG1; HMG3; SBP-1
NCBI Protein Information
high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1
UniProt Protein Name
High mobility group protein B1
UniProt Gene Name
HMGB1
UniProt Synonym Gene Names
HMG1; HMG-1
UniProt Entry Name
HMGB1_HUMAN
