IHC (Immunohistochemistry)
((2.5ug/ml) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining)
Goat RNF213/C17orf27 Polyclonal Antibody | anti-RNF213 antibody
Goat anti-RNF213/C17orf27 (C Terminus) Antibody
Gene Names
RNF213; ALO17; MYMY2; MYSTR; NET57; C17orf27; KIAA1618
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
RNF213/C17orf27; Polyclonal Antibody; Goat anti-RNF213/C17orf27 (C Terminus) Antibody; Goat Anti-RNF213 / C17orf27 (internal) Antibody; RNF213; C17orf27; ring finger protein 213; chromosome 17 open reading frame 27; KIAA1554; RNF213 antibody; C17orf27 antibody; ring finger protein 213 antibody; chromosome 17 open reading frame 27 antibody; KIAA1554 antibody; RNF213 / C17orf27 (C Terminus); anti-RNF213 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
KTAAVLKWNRE
Sequence Length
557
Applicable Applications for anti-RNF213 antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Immunohistochemistry: In paraffin embedded human kidney shows rare staining of nucleoli across the kidney. Recommended concentration, 2.5ug/ml.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 374kDa according to NP_065965.3. Therefore we cannot recommend an optimal concentration and the product is investigative grade.
Immunohistochemistry: In paraffin embedded human kidney shows rare staining of nucleoli across the kidney. Recommended concentration, 2.5ug/ml.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 374kDa according to NP_065965.3. Therefore we cannot recommend an optimal concentration and the product is investigative grade.
Immunogen
Peptide with sequence C-KTAAVLKWNRE, from the C Terminus of the protein sequence according to NP_065965.4.
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
IHC (Immunohistochemistry)
((2.5ug/ml) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining)
IHC (Immunohistochemistry)
((2.5ug/ml) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,260 Da
NCBI Official Synonym Full Names
ring finger protein 213
NCBI Official Symbol
RNF213
NCBI Official Synonym Symbols
ALO17; MYMY2; MYSTR; NET57; C17orf27; KIAA1618
NCBI Protein Information
E3 ubiquitin-protein ligase RNF213
UniProt Protein Name
E3 ubiquitin-protein ligase RNF213
UniProt Gene Name
RNF213
UniProt Entry Name
RN213_HUMAN
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Product Notes
The RNF213 rnf213 (Catalog #AAA13646) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-RNF213/C17orf27 (C Terminus) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNF213/C17orf27 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 4000. Immunohistochemistry: In paraffin embedded human kidney shows rare staining of nucleoli across the kidney. Recommended concentration, 2.5ug/ml. Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 374kDa according to NP_065965.3. Therefore we cannot recommend an optimal concentration and the product is investigative grade. Researchers should empirically determine the suitability of the RNF213 rnf213 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KTAAVLKWNR E. It is sometimes possible for the material contained within the vial of "RNF213/C17orf27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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