Mouse anti-Human, Rat IgA Secretory Component / ECM1 Monoclonal Antibody | anti-ECM1 antibody

IgA Secretory Component / ECM1 Mouse Monoclonal Antibody

Cat. Num. AAA13802

• Gene Names: ECM1; URBWD
• Reactivity: Human, Rat
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: IgA Secretory Component / ECM1, Antibody; IgA Secretory Component / ECM1 Mouse Monoclonal Antibody; ECM1, Extracellular Matrix Protein 1, Secretory Component p85, URBWD; anti-ECM1 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM217
• Specificity: This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 567

• Applicable Applications for anti-ECM1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistology(Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); ; Optimal dilution for a specific application should be determined.
• Cellular Localization: Cell Surface and Cytoplasmic
• Immunogen: Secretory Component protein isolated from human colostrum
• Hu-Chromosome Location: 1q21.2
• Positive Control: Stomach, Lung, or breast tumor
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 degree C to -80 degree C. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified Secretory Component Mouse Monoclonal Antibody (SPM217). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-paraffin human Colon Carcinoma stained with IgA Secretory Component Mab (SPM217).)

References

Bartek J et. al. Histochemistry 91:235-244 (1989)

NCBI and Uniprot Product Information

• NCBI GI #: 322302700
• NCBI GeneID: 1893
• NCBI Accession #: NP_001189787.1
• NCBI GenBank Nucleotide #: NM_001202858.1
• UniProt Accession #: Q16610; O43266; Q5T5G4; Q5T5G5; Q5T5G6; Q8IZ60; A8K8S0; B4DW49; B4DY60
• Molecular Weight: ~80kDa
• NCBI Official Full Name: extracellular matrix protein 1 isoform 3
• NCBI Official Synonym Full Names: extracellular matrix protein 1
• NCBI Official Symbol: ECM1
• NCBI Official Synonym Symbols: URBWD
• NCBI Protein Information: extracellular matrix protein 1
• UniProt Protein Name: Extracellular matrix protein 1
• UniProt Gene Name: ECM1
• UniProt Entry Name: ECM1_HUMAN