Porcine Immunoglobulin A ELISA Kit | IgA elisa kit
Porcine Immunoglobulin A ELISA Kit
Reactivity
Porcine
Synonyms
Immunoglobulin A; N/A; Porcine Immunoglobulin A ELISA Kit; IgA elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of IgA. No significant cross-reactivity or interference between IgA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IgA and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
0.1 mg/ml
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for IgA elisa kit
Principle of the Assay: IgA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IgA antibody and an IgA-HRP conjugate. The assay sample and buffer are incubated together with IgA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgA concentration since IgA from samples and IgA-HRP conjugate compete for the anti-IgA antibody binding site. Since the number of sites is limited, as more sites are occupied by IgA from the sample, fewer sites are left to bind IgA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgA concentration in each sample is interpolated from this standard curve.
Intended Uses: This IgA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine IgA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This IgA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine IgA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for IgA elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
IgA, partial
