Mouse anti-Human, Rat Ring1A Monoclonal Antibody | anti-Ring1A antibody

Anti-Ring1A Mouse mAb

Cat. Num. AAA14160

• Gene Names: RING1; RNF1; RING1A
• Reactivity: Human, Rat
• Applications: Western Blot, Immunofluorescence
• Synonyms: Ring1A; Monoclonal Antibody; Anti-Ring1A Mouse mAb; E3 ubiquitin-protein ligase RING1; Polycomb complex protein RING1; Really interesting new gene 1 protein; RING finger protein 1; Ring1; RING1_HUMAN; RING1A; Rnf1; Transcription repressor Ring1A; anti-Ring1A antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 7D12-G1-B7
• Form/Format: Purified antibody in PBS with 0.05% sodium azide.
• Sequence Length: 406

• Applicable Applications for anti-Ring1A antibody: Western Blot (WB), Immunofluorescence (IF)
• Immunogen: Recombinant fragment corresponding to Human RING1 aa 79-263.
• Sequence Similarities: Contains 1 RING-type zinc finger.
• Subunit Structure: Component of chromatin-associated Polycomb (PcG) complexes. Interacts with BMI1 (By similarity). Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2/RING2 MBLR, L3MBTL2 and YAF2. Interacts with CBX2 and PCGF6. Component of a PRC1-like complex. Component of repressive BCOR complex containing Polycomb group subcomplex at least composed of RYBP, PCGF1, BCOR and RNF2/RING2. Interacts with PCGF2, RNF2; CBX6, CBX7 and CBX8. Interacts with PHC2 (By similarity).
• Subcellular Location: Nucleus speckle

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using Ring1 mouse mAb with DAB staining.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Ring1 mouse mAb with DAB staining.)

FCM (Flow Cytometry)

(Flow cytometric analysis of Hela cells using Ring1 mouse mAb (green) and negative control (red).)

WB (Western Blot)

(Western blot analysis using Ring1 mouse mAb against MOLT-4 (1), LNCaP (2), Hela (3), HEK-293 (4) and Jurkat (5) cell lysate.)

ICC (Immunocytochemistry)

(Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-Ring1A mouse mAb (dilution 1:200).)

WB (Western Blot)

(Western blot analysis of extracts from Jurkat, A549, MCF7 and C6 cell lysates using Ring1A mouse mAb (1:3000 diluted).Predicted band size:42KDa.Observed band size:54KDa.)

Related Product Information for anti-Ring1A antibody

Entrez Summary: This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq, Jul 2008]
UniPort Summary Function: Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of Lys-119 of histone H2A, thereby playing a central role in histone code and gene regulation. H2A Lys-119 ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.

NCBI and Uniprot Product Information

• NCBI GI #: 51479192
• NCBI GeneID: 6015
• NCBI Accession #: NP_002922.2
• NCBI GenBank Nucleotide #: NM_002931.3
• UniProt Accession #: Q06587; Q5JP96; Q5SQW2; Q86V19; A8JZZ0
• Molecular Weight: 54kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase RING1
• NCBI Official Synonym Full Names: ring finger protein 1
• NCBI Official Symbol: RING1
• NCBI Official Synonym Symbols: RNF1; RING1A
• NCBI Protein Information: E3 ubiquitin-protein ligase RING1
• UniProt Protein Name: E3 ubiquitin-protein ligase RING1
• UniProt Gene Name: RING1
• UniProt Synonym Gene Names: RNF1