SDS-PAGE
(The purity of CAMK2γ was determined to be >75% by densitometry. CAMK2γ Approx. MW 86kDa.)
CAMK2 gamma Recombinant Protein | CAMK2gamma recombinant protein
CAMK2 gamma, Active
Applications
Western Blot
Purity
>75%
Synonyms
CAMK2 gamma; Active; CAMKG; CAMK; CAMK-II; MGC26678; CAMK2G; CAMK2gamma recombinant protein
Host
Sf9 insect cells
Purity/Purification
>75%
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Applicable Applications for CAMK2gamma recombinant protein
Kinase Assay, Western Blot (WB)
Specific Activity
260nmol/min/mg
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(The purity of CAMK2γ was determined to be >75% by densitometry. CAMK2γ Approx. MW 86kDa.)
SDS-PAGE
(The purity of CAMK2γ was determined to be >75% by densitometry. CAMK2γ Approx. MW 86kDa.)
Activity
(The specific activity of CAMK2g was determined to be 260 nmol/min/mg as per activity assay protocol.)
Activity
(The specific activity of CAMK2g was determined to be 260 nmol/min/mg as per activity assay protocol.)
Related Product Information for CAMK2gamma recombinant protein
Full-length recombinant human CAMK2g was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. The gene accession number is NM_172169.
CAMK2gamma is a member of the CAMKII family which are ubiquitous serine/threonine protein kinases that have been implicated in diverse effects of hormones and neurotransmitters. CAMK2gamma has six alternatively spliced variants that encode six different isoforms. Some of these variants have been identified in human tumors (1). Transgenic mice expressing a partially calcium-independent mutant form of CAMK2gamma showed 1.5-to 2-fold increase in the thymus of these mice, at least in part due to an increase in the life span of double-positive thymocytes (2). There was an increase in the number of T cells in the secondary lymphoid organs that had acquired an antigen-dependent memory phenotype.
CAMK2gamma is a member of the CAMKII family which are ubiquitous serine/threonine protein kinases that have been implicated in diverse effects of hormones and neurotransmitters. CAMK2gamma has six alternatively spliced variants that encode six different isoforms. Some of these variants have been identified in human tumors (1). Transgenic mice expressing a partially calcium-independent mutant form of CAMK2gamma showed 1.5-to 2-fold increase in the thymus of these mice, at least in part due to an increase in the life span of double-positive thymocytes (2). There was an increase in the number of T cells in the secondary lymphoid organs that had acquired an antigen-dependent memory phenotype.
Product Categories/Family for CAMK2gamma recombinant protein
References
1. Tombes, R. M. et al: Identification of novel human tumor cell-specific CaMK-II variants. Biochim. Biophys. Acta 1355: 281-292, 1997.
2. Bui, J. D. et al: A role for CaMKII in T cell memory. Cell 100: 457-467, 2000.
2. Bui, J. D. et al: A role for CaMKII in T cell memory. Cell 100: 457-467, 2000.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The CAMK2gamma (Catalog #AAA14262) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CAMK2 gamma can be used in a range of immunoassay formats including, but not limited to, Kinase Assay, Western Blot (WB). Researchers should empirically determine the suitability of the CAMK2gamma for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAMK2 gamma, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
