Standard Curve (Sample)
Interleukin-2 ELISA Kit | IL-2 elisa kit
Interleukin-2 (IL-2)
Gene Names
IL2; IL-2; TCGF; lymphokine
Synonyms
Interleukin-2; Interleukin-2 (IL-2); IL-2 elisa kit
Specificity
This sandwich ELISA can detect both natural and recombinant human IL-2. This kit exhibits no significant cross-reactivity with human IL-1, IL-3, IL-4, IL-6, IL-7, IL-8, G-CSF, GM-CSF, TGF-beta1, TGF-beta2, TNF-alpha, TNF-beta.
Sequence Length
153
Samples
Serum, plasma, cell culture supernatant, and other biological fluids
Assay Type
Quantitative Sandwich
Intra-assay Precision
To determine within-run precision, three different samples of known concentration were assayed by replicates of twenty in 1 assay.
Inter-assay Precision
To determine between-run precision, three different samples of known concentration were assayed by replicates on 20 different assays.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL-2 elisa kit
Intended Uses: This Human Interleukin 2 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 2 (IL-2) concentrations in serum, plasma, cell culture supernatant, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH USE ONLY.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for IL-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and colour develops in proportion to the amount of IL-2 bound in the initial step. The colour development is stopped and the intensity of the colour is measured. In order to measure the concentration of IL-2 in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-2 concentration (pg/mL). The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Background/Introduction: Interleukin 2 (IL-2) is a lymphokine synthesized and secreted primarily by T helper lymphocytes that have been activated by stimulation with certain mitogens or by interaction of the T cell receptor complex with antigen/MHC complexes on the surfaces of antigen-presenting cells. The response of T helper cells to activation is induction of the expression of IL-2 and receptors for IL-2 and, subsequently, clonal expansion of antigenspecific T cells. At this level IL-2 is an autocrine factor, driving the expansion of the antigen-specific cells. IL-2 also acts as a paracrine factor, influencing the activity of other cells, both within the immune system and outside of it. B cells and natural killer (NK) cells respond to IL-2 when properly activated. The so-called lymphocyte activated killer, or LAK cells, appears to be derived from NK cells under the influence of IL-2. Human IL-2 is a glycoprotein with an apparent molecular weight of 15,000 - 18,000. Natural IL-2 is glycosylated and varying degrees of glycosylation apparently account for the observed range of molecular weights seen on SDS-PAGE. Human IL-2 is synthesized as a polypeptide of 153 amino acid residues. The first 20 amino acids represent a signal sequence that is cleaved to produce the mature factor. The mature protein contains three cysteine residues, two of which form a disulfide bond that is required for biological activity. Murine IL-2 is approximately 63% identical to human IL-2, but contains a unique stretch of repeated glutamine residues. There is marked species cross-reactivity as human IL-2 has been found to be active on murine cell lines. Cells known to produce IL-2 include thymocytes, gamma delta T cells, B cells, CD4+ and CD8+ T cells, and neurons plus astrocytes. IL-2 is a factor produced and secreted primarily by activated T helper cells that acts as a autocrine factor driving the expansion of antigen-specific cells and as a paracrine factor influencing the activity of a number of other cells including B cells, NK cells and LAK cells. A simplified but useful view of these activities is of lymphocytes expanding under the influence of IL-2 and becoming the target of other cytokines that cause their functional differentiation. With respect to the specific role of IL-2 on the differentiation of T cells, the separation of CD4+ T helper cells into the categories of TH1 and TH2 according to their function in cell mediated or humoral immunity is a concept that is proving useful. In this system each category of cells secretes a characteristic set of cytokines that functions as a network to push the system either towards cellular immunity (delayed type hyper-sensitivity and cellular cytotoxicity) associated with TH1, or towards humoral immunity (antibodymediated) associated with TH2. IL-2, along with IFN-gamma and TNF-beta, is a defining product of the TH1 subset. Although the TH1 and TH2 subsets are relatively clearly defined in the murine immune system, these categories are not so clear-cut in the human immune system where the designations TH1-like and TH2-like have been suggested. Other cells under the possible influence of IL-2 are neutrophils, monocytes, and gamma delta T cells, all of which demonstrate either activation, augmented function, or increased survival when exposed to IL-2. Finally, it should be mentioned that IL-2 is finding its way, along with many other cytokines, into the neurosciences as a possible neuromodulator and growth regulator of glial cells. Because of the central role of the IL-2/IL-2R system in mediation of the immune response, it is obvious that monitoring and manipulation of this system has important test and therapeutic implications. IL-2 has shown promise as an anti-cancer drug by virtue of its ability to stimulate the proliferation and activities of tumor-attacking LAK and TIL (tumorinfiltrating lymphocytes) cells. However, problems with IL-2 toxicity are still of concern and merit investigation.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-2 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for IL-2 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and colour develops in proportion to the amount of IL-2 bound in the initial step. The colour development is stopped and the intensity of the colour is measured. In order to measure the concentration of IL-2 in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-2 concentration (pg/mL). The concentration of IL-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Background/Introduction: Interleukin 2 (IL-2) is a lymphokine synthesized and secreted primarily by T helper lymphocytes that have been activated by stimulation with certain mitogens or by interaction of the T cell receptor complex with antigen/MHC complexes on the surfaces of antigen-presenting cells. The response of T helper cells to activation is induction of the expression of IL-2 and receptors for IL-2 and, subsequently, clonal expansion of antigenspecific T cells. At this level IL-2 is an autocrine factor, driving the expansion of the antigen-specific cells. IL-2 also acts as a paracrine factor, influencing the activity of other cells, both within the immune system and outside of it. B cells and natural killer (NK) cells respond to IL-2 when properly activated. The so-called lymphocyte activated killer, or LAK cells, appears to be derived from NK cells under the influence of IL-2. Human IL-2 is a glycoprotein with an apparent molecular weight of 15,000 - 18,000. Natural IL-2 is glycosylated and varying degrees of glycosylation apparently account for the observed range of molecular weights seen on SDS-PAGE. Human IL-2 is synthesized as a polypeptide of 153 amino acid residues. The first 20 amino acids represent a signal sequence that is cleaved to produce the mature factor. The mature protein contains three cysteine residues, two of which form a disulfide bond that is required for biological activity. Murine IL-2 is approximately 63% identical to human IL-2, but contains a unique stretch of repeated glutamine residues. There is marked species cross-reactivity as human IL-2 has been found to be active on murine cell lines. Cells known to produce IL-2 include thymocytes, gamma delta T cells, B cells, CD4+ and CD8+ T cells, and neurons plus astrocytes. IL-2 is a factor produced and secreted primarily by activated T helper cells that acts as a autocrine factor driving the expansion of antigen-specific cells and as a paracrine factor influencing the activity of a number of other cells including B cells, NK cells and LAK cells. A simplified but useful view of these activities is of lymphocytes expanding under the influence of IL-2 and becoming the target of other cytokines that cause their functional differentiation. With respect to the specific role of IL-2 on the differentiation of T cells, the separation of CD4+ T helper cells into the categories of TH1 and TH2 according to their function in cell mediated or humoral immunity is a concept that is proving useful. In this system each category of cells secretes a characteristic set of cytokines that functions as a network to push the system either towards cellular immunity (delayed type hyper-sensitivity and cellular cytotoxicity) associated with TH1, or towards humoral immunity (antibodymediated) associated with TH2. IL-2, along with IFN-gamma and TNF-beta, is a defining product of the TH1 subset. Although the TH1 and TH2 subsets are relatively clearly defined in the murine immune system, these categories are not so clear-cut in the human immune system where the designations TH1-like and TH2-like have been suggested. Other cells under the possible influence of IL-2 are neutrophils, monocytes, and gamma delta T cells, all of which demonstrate either activation, augmented function, or increased survival when exposed to IL-2. Finally, it should be mentioned that IL-2 is finding its way, along with many other cytokines, into the neurosciences as a possible neuromodulator and growth regulator of glial cells. Because of the central role of the IL-2/IL-2R system in mediation of the immune response, it is obvious that monitoring and manipulation of this system has important test and therapeutic implications. IL-2 has shown promise as an anti-cancer drug by virtue of its ability to stimulate the proliferation and activities of tumor-attacking LAK and TIL (tumorinfiltrating lymphocytes) cells. However, problems with IL-2 toxicity are still of concern and merit investigation.
Product Categories/Family for IL-2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
17,628 Da
NCBI Official Full Name
interleukin-2
NCBI Official Synonym Full Names
interleukin 2
NCBI Official Symbol
IL2
NCBI Official Synonym Symbols
IL-2; TCGF; lymphokine
NCBI Protein Information
interleukin-2; T cell growth factor; aldesleukin; involved in regulation of T-cell clonal expansion
UniProt Protein Name
Interleukin-2
UniProt Gene Name
IL2
UniProt Entry Name
IL2_HUMAN
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Product Notes
The IL-2 il2 (Catalog #AAA14616) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Interleukin-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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