WB (Western Blot)
(Western Blot under reducing conditions of human ovarian cancer tissue lysates using AAA14734 2ug/ml. Followed by IgG (HRP) Anti-Mouse. A specific band was detected for Mesothelin at approximately 50kD.)
Mouse anti-Human MSLN Monoclonal Antibody | anti-MSLN antibody
MSLN (Mesothelin, CAK1 Antigen, MPF, Pre-pro-megakaryocyte-potentiating Factor, SMR)
Gene Names
MSLN; MPF; SMRP
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
MSLN; Monoclonal Antibody; MSLN (Mesothelin; CAK1 Antigen; MPF; Pre-pro-megakaryocyte-potentiating Factor; SMR); Anti -MSLN (Mesothelin; anti-MSLN antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10K75
Specificity
Recognizes human Mesothelin. Does not recognize recombinant MPF.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS,pH7.4, 5% trehalose. Reconsitute with 200ul sterile PBS.
Concentration
~0.5mg/ml (after reconstitution) (varies by lot)
Applicable Applications for anti-MSLN antibody
ELISA (EL/EIA), Western Blot (WB), Simple Western (SW)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 2ug/ml
Simple Western: 20ug/ml
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 2ug/ml
Simple Western: 20ug/ml
Optimal dilutions to be determined by the researcher.
Immunogen
Purified, recombinant protein corresponding to aa296-580 from, human Mesothelin, expressed in NSO cells
Acession #
Q13421
Hybridoma
Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human MPF.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
WB (Western Blot)
(Western Blot under reducing conditions of human ovarian cancer tissue lysates using AAA14734 2ug/ml. Followed by IgG (HRP) Anti-Mouse. A specific band was detected for Mesothelin at approximately 50kD.)
WB (Western Blot)
(Western Blot under reducing conditions of human ovarian cancer tissue lysates using AAA14734 2ug/ml. Followed by IgG (HRP) Anti-Mouse. A specific band was detected for Mesothelin at approximately 50kD.)
Related Product Information for anti-MSLN antibody
Megakaryocyte Potentiating Factor (MPF) is a 33kD soluble protein that is cleaved as an N-terminal propeptide from the mouse mesothelin preproprotein by furin, while the C-terminal portion is processed further to form mesothelin. Mouse MPF potentiates megakaryocyte colony forming activity of IL-3 in mouse bone marrow culture. Human MPF shows 55% aa identity with mouse or rat MPF. The preproprotein is produced by mesothelial cells and is also highly expressed in pancreatic, gastric and ovarian carcinomas.
Product Categories/Family for anti-MSLN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
mesothelin isoform 1 preproprotein
NCBI Official Synonym Full Names
mesothelin
NCBI Official Symbol
MSLN
NCBI Official Synonym Symbols
MPF; SMRP
NCBI Protein Information
mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor
UniProt Protein Name
Mesothelin
UniProt Gene Name
MSLN
UniProt Synonym Gene Names
MPF; MPF
UniProt Entry Name
MSLN_HUMAN
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Product Notes
The MSLN msln (Catalog #AAA14734) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MSLN (Mesothelin, CAK1 Antigen, MPF, Pre-pro-megakaryocyte-potentiating Factor, SMR) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MSLN can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Simple Western (SW). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 2ug/ml Simple Western: 20ug/ml Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the MSLN msln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MSLN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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