Mouse Mucin-5 subtype AC, MUC5AC ELISA Kit | MUC5AC elisa kit
Mouse Mucin-5 subtype AC, MUC5AC ELISA Kit
Reactivity
Mouse
Synonyms
Mucin-5 subtype AC, MUC5AC; N/A; Mouse Mucin-5 subtype AC, MUC5AC ELISA Kit; Mouse Mucin-5 subtype AC; MUC5AC ELISA Kit; Mucin-5 subtype AC; MUC5AC; MUC5AC elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse MUC5AC. No significant cross-reactivity or interference between mouse MUC5AC and analogues was observed.
Sequence Length
67
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
4.688 ng/ml-300 ng/ml
Sensitivity
< 1.172 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for MUC5AC elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUC5AC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC5AC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC5AC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC5AC bound in the initial step. The color development is stopped and the intensity of the color is measured.
