Rat Mucin-5 subtype AC ELISA Kit | MUC5AC elisa kit

Rat Mucin-5 subtype AC ELISA Kit

Cat. Num. AAA16502

• Reactivity: Rat
• Synonyms: Mucin-5 subtype AC; N/A; Rat Mucin-5 subtype AC ELISA Kit; MUC5AC elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of MUC5AC. No significant cross-reactivity or interference between MUC5AC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MUC5AC and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive or Sandwich
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for MUC5AC elisa kit

Principle of the Assay: MUC5AC ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MUC5AC antibody and an MUC5AC-HRP conjugate. The assay sample and buffer are incubated together with MUC5AC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MUC5AC concentration since MUC5AC from samples and MUC5AC-HRP conjugate compete for the anti-MUC5AC antibody binding site. Since the number of sites is limited, as more sites are occupied by MUC5AC from the sample, fewer sites are left to bind MUC5AC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MUC5AC concentration in each sample is interpolated from this standard curve.

Intended Uses: This MUC5AC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat MUC5AC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for MUC5AC elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 15026993
• Molecular Weight: 6,501 Da
• NCBI Official Full Name: MUC5AC protein, partial
• UniProt Protein Name: MUC5AC protein
• UniProt Gene Name: MUC5AC
• UniProt Entry Name: Q96DU6_HUMAN