Human 8-iso prostaglandin F2alpha, 8-iso-PGF2a ELISA Kit | 8-iso-PGF2a elisa kit
Human 8-iso prostaglandin F2alpha, 8-iso-PGF2a ELISA Kit
Reactivity
Human
Synonyms
8-iso prostaglandin F2alpha, 8-iso-PGF2a; N/A; Human 8-iso prostaglandin F2alpha, 8-iso-PGF2a ELISA Kit; Human 8-iso prostaglandin F2alpha; 8-iso-PGF2a ELISA Kit; 8-iso prostaglandin F2alpha; 8-iso-PGF2a; 8-iso-PGF2a elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human 8-iso-PGF2a. No significant cross-reactivity or interference between human 8-iso-PGF2a and analogues was observed.
Assay Type
Competitive
Samples
Serum, plasma
Detection Range
125 pg/ml-5000 pg/ml
Sensitivity
< 125pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for 8-iso-PGF2a elisa kit
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for 8-iso-PGF2a and Horseradish Peroxidase (HRP) conjugated 8-iso-PGF2a. The competitive inhibition reaction is launched between with HRP labeled 8-iso-PGF2a and unlabeled 8-iso-PGF2a with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of 8-iso-PGF2a in the sample. The color development is stopped and the intensity of the color is measured.